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On the substrate- and stereospecificity of the plant carotenoid cleavage dioxygenase 7

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On the substrate- and stereospecificity of the plant carotenoid cleavage dioxygenase 7

by Mark Bruno, Manuel Hofmann, Martina Vermathen, Adrian Alder, Peter Beyer, Salim Al-Babili
Scientific Year: 2014 ISSN: 0036-8075 DOI: 10.1016/j.febslet.2014.03.041

Bibliography

Mark Bruno, Manuel Hofmann, Martina Vermathen, Adrian Alder, Peter Beyer, Salim Al-Babili​. On the substrate- and stereospecificity of the plant carotenoid cleavage dioxygenase 7. FEBS Letters. https://doi.org/10.1016/j.febslet.2014.03.041

Abstract

Strigolactones are phytohormones synthesized from carotenoids via a stereospecific pathway involving the carotenoid cleavage dioxygenases 7 (CCD7) and 8. CCD7 cleaves 9-cis-β-carotene to form a supposedly 9-cis-configured β-apo-10′-carotenal. CCD8 converts this intermediate through a combination of yet undetermined reactions into the strigolactone-like compound carlactone. Here, we investigated the substrate and stereo-specificity of the Arabidopsis and pea CCD7 and determined the stereo-configuration of the β-apo-10′-carotenal intermediate by using Nuclear Magnetic Resonance Spectroscopy. Our data unequivocally demonstrate the 9-cis-configuration of the intermediate. Both CCD7s cleave different 9-cis-carotenoids, yielding hydroxylated 9-cis-apo-10′-carotenals that may lead to hydroxylated carlactones, but show highest affinity for 9-cis-β-carotene.

Keywords

apocarotenoid carlactone carotenoid carotenoid cleavage dioxygenase strigolactone
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