This chapter details the methodology for collecting and processing plant root exudates using a C18 solid-phase extraction column, followed by the isolation, identification, and quantification of strigolactones (SLs) through liquid chromatography-mass spectrometry (LC-MS).
Keywords: C18 solid-phase extraction column; Liquid chromatography with tandem mass spectrometry (LC-MS/MS); Liquid chromatography-mass spectrometry (LC-MS); Pearl millet; Rhizosphere signals; Rice; Root exudates; Sorghum; Strigolactones.